High Performance Liquid Chromatography (HPLC)
LOW PRESSURE GRADENT MIXING
Q–ADEPT HPLC SYSTEM Q-2, Binary – Low Pressure Gradient
This is a low pressure binary gradient forming system. It uses the CE 4102 dual piston pump with flow rates from 0.001 to 10mL/min and a maximum operating pressure of 40MPa.
The pump has the facility for mixing two solvents for binary gradients and of course may be used in the isocratic mode.The Adept CE 4200 dual wavelength detector is used with an 8µL x 10mm flowcell. The Rheodyne valve fitted with a 20µL loop is mounted with the column on the mounting panel CE 4050.The PowerStream software controller is mounted at the centre of the stack. Full control and data processing are provided for use with Windows.
A Solvent Reservoir and Pipe Organizer and installation kit are also supplied.
Q-ADEPT HPLC SYSTEM Q-4, Quaternary – Low Pressure Gradient
This extremely popular quaternary low pressure gradient system is shown here with a Cecil column heater/chiller, which should be ordered extra if required. The CE 4104 dual piston pump has flow rates from 0.001 to 10µL/min and a maximum operating pressure of 40MPa. The pump has all the facilities for gradient formation, with low pressure mixing of up to 4 solvents.
Four, proprietary, high performance, high reliability, proportioning valves are used to control up to four solvents and a high performance, low volume, dynamic mixer is used for ultimate gradient quality.
The Adept CE 4200 dual wavelength detector is used with an 8µL x 10mm flowcell. The Rheodyne valve with 20µL loop is mounted by a small panel to the detector. When an oven is not used the column may be mounted together with the valve on mounting panel CE 4050.
The CE 4900 Power Stream system controller is placed at the center of the very compact stack. Full control and data processing are provided for use in Windows. The system is supplied complete with solvent and pipe organizer, CE 4134, 8µL x 10mm flow cell and installation kit 4150 14 00.
Q-ADEPT HPLC SYSTEM Q-4S, Method Development
This is the ideal system for sophisticated method development. The system offers power and flexibility and yet is compact and convenient to use.
The CE 4104 Quaternary pump is provided for low pressure mixing of up to 4 solvents. A dynamic mixer, built into the pump, provides excellent solvent mixing for reproducible gradients.
The Adept CE 4300 WaveQuest DAD fast scanning detector is supplied with an 8µL x 10mm flowcell and fitted with a mounting panel for the Rheodyne injection valve.
Purity, compound identification, match factors using library search and virtually drift free monitoring may be carried out using this excellent system on the same scan as peaks are quantified cutting experimental time in half.
The PowerStream system controller is mounted at the centre of the stack and full control and data Processing are provided for use in Windows. A solvent and pipe organiser, CE 4134 and installation kit 4150 14 00 are provided.
The system is completed by the CE 4600 heated and cooled oven, which accommodates up to three columns.
HIGH PRESSURE GRADIENT MIXING
ADEPT HPLC SYSTEM 1, Analytical Isocratic
This simple system is ideal for routine isocratic separations and quality assurance applications. High sensitivity is provided by the Adept CE 4201 UV-Visible variable wavelength detector with 8µL x 10mm flowcell. Precise delivery of the mobile phase is provided by the Adept CE 4100 dual piston high pressure solvent delivery pump which delivers flow rates from 0.001 to 10mL/min with a maximum operating pressure of 40MPa. The pump head is constructed with ceramic inserts to provide bio- compatibility.
The system may be upgraded to provide data processing and is supplied with column mounting panel and cover and Rheodyne injection valve with a 20µL loop, solvent and pipe organiser CE 4033, 8µL x 10mm flowcell and installation kit 4150 01 00.
ADEPT HPLC SYSTEM 2, Analytical Isocratic with Data Processing
This system uses the Adept CE 4100 dual piston pump and the Adept CE 4200 dual wavelength detector for added flexibility and is provided with PowerStream data processing software and interface unit, CE 4901.
PowerStream software, CE 4901, operates in Windows. It offers the same data processing power as the CE 4900 software but unlike the latter, offers no system control. The system is supplied complete with column and valve mounting panel, Rheodyne injection valve with 10µL loop, solvent and pipe organiser CE 4033, 8µL x 10mm cell and installation kit 4150 01 00.
ADEPT HPLC SYSTEM 3, Automatic Analytical Isocratic
Routine analysis of large numbers of samples may be carried out using this system which requires minimum operator attention. System control and data processing are provided by PowerStream software and interface CE 4900. The elegant, powerful and very easy to use software operates in Windows.
A range of autosamplers accommodates large numbers of samples. The system, like all the Adept systems, uses the CE 4100 dual piston pump with delivery speeds from 0.001 to 10mL/min.
The CE 4200 dual wavelength detector has a very low noise level, less than ± 0.35 x 10-5A peak to peak and incredibly low drift ± 3 x 10-5A/h at temperature equilibrium. The 8µL x 10mm flow cell, column panel and cover, solvent and pipe organizer CE 4033 and installation kit 4150 01 01 are provided.
ADEPT HPLC SYSTEM 4, Binary Gradient – High Pressure Gradient
Two Adept CE 4100 dual piston pumps, with flow rates from 0.001 to 10mL/min and a maximum operating pressure of 40MPa, are used with the dynamic mixer CE 4120.
The Adept dual wavelength UV-Visible detector CE 4200 is used with an 8µL x 10mm flow cell. A column mounting panel, cover and Rheodyne valve with a 20µL loop are provided.
The software provides full system control and data processing using Windows. The system is supplied with solvent and pipe organizer CE 4033 and installation kit.
HPLC Pump CE 4100
| HPLC Pump CE 4100 | |
| Flow Rate | 0.001 to 10mL/min in 0.001 steps |
| Flow Reproducibility | Better than 0.1% |
| Maximum Pressure | 40MPa = 400 bar |
| User Interface | 4 line back-lit display
Full range of keys for menu and parameter selection Fast number entry keys |
| Over Pressure Cut-out | Adjustable between 3 and 40MPa |
| Low Pressure Cut-out | Adjustable between 0 and 40MPa |
| Material in contact with fluids | Ceramic inserts – biocompatible
Check valves – ruby and sapphire |
| Pulsation | Less than ±0.5% at 2mL/min |
| Size and Weight | 365 x 290 x 140mm, 7.5kg |
| Power Requirements | 115V or 230V, 50/60Hz, 30VA |
Quaternary Low Pressure Pump CE 4104
| Quaternary Low Pressure Pump CE 4104 | |
| Flow Rate | 0.001 to 10mL/min in 0.001/min increments |
| Pressure Range | 0-40MPa over flow range |
| Flow Accuracy | <±0.5% |
| Flow Precision | <0.1% RSD |
| Pressure Pulsation | <±0.5% at 2mL/min |
| Gradient Forming | Quaternary to Binary low pressure mixing and isocratic high pressure operation |
| Gradient Control | Using proprietary high-speed proportioning valves |
| Gradient Precision | Typically 0.2% RSD at 1mL/min |
| Material in contact with fluids | Ceramic inserts, ruby and sapphire check valves, PEEK |
| Gradient Forming | Binary gradient low pressure mixing and isocratic high pressure operation |
Dual Wavelength, Programmable UV Visible Detector CE 4200
| Dual Wavelength, Programmable UV Visible Detector CE 4200 | |
| Noise | ±0.35 x 10-5 A at 230nm, 2s, using air test cell |
| Drift | ±0.3 x 10-4 A at 230nm, 2s, using air test cell |
| Dual Wavelength Operation | Simultaneous measurements at 2 wavelengths
Output of Absorbance ratio for the 2 wavelengths |
| Noise, Dual Wavelength | ± 2 x 10-5 A peak to peak 230/280nm 1s time constant |
| Drift, Dual Wavelength | ± 1 x 10-4 A/hour at constant temperature |
| Programmed Operation | Programs may be entered to change parameters, operating modes and select up to 6 different wavelengths in any time sequence. |
UV-Visible Detector CE 4201
| UV-Visible Detector CE 4201 | |
| Optical System | Double beam with 1200 lines/mm grating monochromator |
| Wavelength Range | 190-700nm |
| Wavelength Accuracy | ± 1nm |
| Wavelength Precision | ± 0.1nm, single wavelength mode |
| Optical Bandwidth | 8nm |
| Straylight | < .02%, 220nm |
| Lamp | Deuterium lamp with elapsed time indicator |
| Absorbance Range | 0.001 to 2A full scale in 11 ranges |
| Time Constant | 0.1, 0.2, 0.5, 1, 2, 5 and 10 seconds |
| Display | Four-line display of parameters, menus, prompts, wavelength, Absorbance etc. |
| Fast Number | Entry By use of special number keys |
| Event Marking | From keypad or by external source |
| Method Storage | For up to 30 methods, security protected |
| Second Order Filter | Fast automatic insertion at 370nm |
| Noise | ± 1 x 10-5 A peak to peak, 220nm, 2s time constant |
| Drift | ± 1 x 10-4 A/hour at constant temperature |
| Linearity | Better than 1%, 0.001 – 2A |
| Automatic Power-up/off | Pre-programmed from keypad to save warm up time |
| Sample Flow Cell | 8µL x 10mm pathlength – normal
4µL x 5mm pathlength Wetted surfaces PEEK, Teflon and Fused Silica Other cells available |
| Refractive Index Sensitivity | < ± 5 x 10-4 A, water to methanol at 350nm |
| Flow Rate Sensitivity | < ± 3.5 x 10-3 A, for flow change 1-2mL/min, 300nm |
| Outputs | Two Absorbance outputs and Absorbance ratio to recorder. All data available to PowerStream System Manager. |
WaveQuest D.A.D. Ultra Fast Scanning Detector CE 4300
| WaveQuest D.A.D. Ultra Fast Scanning Detector CE 4300 | |
| Optical System | Double beam |
| Monochromator | With 1200 l/mm holographic grating |
| Wavelength Range | 190-700nm |
| Optical Bandwidth | 4nm – constant |
| Digital Resolution | 1nm |
| Wavelength Accuracy | 1nm |
| Wavelength Reproducibility | 0.1nm |
| Absorbance Range | 0 to 3A |
| Scan Speeds | Up to 1000nm/s |
| Baseline Flatness | ± 0.002A over most of range |
| Straylight | <0.02% at 220nm and 340nm |
| Noise | < ±0.5 x 10–5 A at 230nm, 2s air test cell |
| Drift | < ±0.5 x 10–4 A at 230nm, using air test cell |
| Calibration and Test | Automatic at switch-on |
| Display | Four line display of parameters, menus, prompts, wavelength, Absorbance etc. |
| Number of Peak Scans | Up to 100 peaks for any single elution |
| Multiple Scans of a Peak | Up to 8 scans of any peak |
| Scan Trigger | At start, set Absorbance value, point of inflection or at maximum |
| Scan Storage | Up to 100, password protected, unlimited with PowerStream |
| Method Storage | Up to 30 methods, password protected |
| Size | 365 x 290 x 140mm, 17Kg |
| Power Requirement | 115V or 250V, 50/60Hz, 50VA |
Refractive Index Detector CE 4700
| Refractive Index Detector CE 4700 | |
| Measuring Ranges | ± 10-4 RIU |
| Sensitivity | 8 x 10-8 RIU |
| Noise | 8 x 10-8 RIU |
| Time constants | 0.1, 0.2, 0.5, 1, 2, 5, 10 seconds |
| Cell volume | 9µL |
| Outputs | -1V to +1V, 20 bit resolution |
| Size and weight | 360 x 140 x 260mm, 9.5kg |
| Power requirements | 100-250V, 50/60Hz, 6VA |
Fluorescence Detector Dual Monochromator CE 4500
| Fluorescence Detector Dual Monochromator CE 4500 | |
| Light Source | 10W Xenon flash lamp, frequency programmable |
| Detector | Photomultiplier |
| Excitation Monochromator | 200-650nm |
| Emission Monochromator | 200-900nm, with extended range photomultiplier |
| Wavelength Scanning | Excitation and emission independently programmable |
| Sensitivity | S/N >3000 for 4µg/L Anthracene in Methanol |
| Analogue Outputs | 10mV, 100mV and 1V |
| Power Requirements | 100/240V, 50/60Hz |
Conductivity Detector CE 4710
| Conductivity Detector CE 4710 | |
| Conductance Display Range | 0.01 – 5000µS. cm-1 |
| Time Constant | 0.1, 0.2, 0.5, 1, 1.5, 2, 5s |
| Autozero Range | Typically up to 10,000µS. cm-1 |
| Synchronous Detection Frequency | 5KHz |
| Noise | Less than ±1nS/cm, DI water, 1mL/min, 2s |
| Drift | Less than ±10nS/cm/h, DI water, 1mL/min, |
| Cell Volume | 0.5µL |
| Cell Body | PEEK P1000 grade |
| Electrodes | 316 stainless steel |
| Cell Pressure Rating | 200psi |
| Operating Temperature | 25 – 50ºC; 35ºC standard |
| Temperature Precision | ± 0.01ºC |
| Warm-up Time | 10 minutes typical |
| Method Storage | Up to 100 with password protection |
| Outputs | 0-1V analogue and RS232 |
| Digital Control | By PowerStream software |
Electrochemical Detector CE 4720
| Electrochemical Detector CE 4720 | |
| Working electrode | Glassy carbon (optional solid Ag, Au, Cu, Ni, Pt electrodes) |
| Auxiliary electrode | Stainless steel |
| Reference electrode | Silver/silver chloride salt bridge electrode or embedded silver electrode |
| Cell volume | 120 nl with 50 µm spacer |
| Time Constant | 0.1, 0.2, 0.5, 1, 1.5, 2, 5s |
| Noise | Less than ±0.3 pA |
| Drift | Less than 0.3 pA |
| Cell Pressure Rating | 200 psi |
| Cell Enclosure Heater | 20 watt |
| Operating Temperature | 25 – 50ºC; 35ºC standard |
| Temperature Precision | ± 0.01ºC |
| Warm-up Time | 10 minutes typical |
| LCD Screen | 4 lines, back lit, adjustable contrast |
| Method Storage | Up to 100 with password protection |
| Analogue Output | 0-1V |
| Remote Autozero | By contact closure |
| Keypad Control | For entry of values, commands and Programmes |
| Working potential | ±2.5 V |
| Measuring range | ±10 pA to 5 mA |
| Size & Weight | 365 x 290 x 140 mm, 7.5kg |
| Power Requirements | 115V or 230V, 50/60Hz, 40 Watt |